Figure 7.

CB002 treatment reduces the stability of the R175H p53 mutant. A. CB002 reduces the protein expression of the exogenous R175H mutant in HCT116 p53-null cells and not the HCT116 wild-type p53 cells. B. HCT116 R175H p53 cells were treated for 24 hrs with DMSO or CB002 followed by 100 μg/mL cycloheximide addition, protein stability was evaluated from 0 - 10 hrs. C. CB002 reduces the protein expression of the endogenous R175H mutant p53 in RXF393 renal cancer cells. D. Co-treatment of 24 hrs with proteasome inhibitor MG132 and CB002 rescues the expression of the R175H mutant p53, suggesting the involvement of the ubiquitin proteasome system in CB002-mediated mutant p53 degradation.