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. 2018 Feb 19;17(5):557–567. doi: 10.1080/15384101.2017.1346762

Figure 8.

Figure 8.

CB002-mediated autophagy contributes to apoptotic cell death in drug-treated cells. CB002 induces autophagy as indicated by A. Specific recognition of autophagic vacuoles (detection using the CYTO-ID® autophagy detection kit at 24 hrs) and B. LC3B protein expression levels in DLD-1 cells (cells were treated with 160 μM CB002 or DMSO control for a period of 2–48 hrs. C. Autophagy inhibition by Chl does not rescue mutant p53 protein expression in HCT116 R175H p53 D. Blocking the autophagy process in SW480 cells with 50 μM chloroquine (Chl) reduces the expression of apoptotic markers cleaved caspase 8 (c-Caspase 8) and cleaved PARP (24 hrs) E. Inhibition of autophagy by Atg5 siRNA completely abolishes PARP cleavage in SW480 cells. F. Atg5-FADD apoptosis axis is not involved in CB002 mediated apoptosis in SW480 cells (24 hrs).