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. 2018 Apr 5;17(5):535–549. doi: 10.1080/15384101.2018.1442621

Figure 6.

Figure 6.

JARID2 KO phenotype in naïve hESC. (A) Western blot analysis of EZH2, OCT4, NANOG, and H3K27Me3 marks in JARID2 Knockout clones of 2iL-I-F naïve hESC. (B) Schematic representation of mutation in JARID2 KO Clone2. (C-D) Confocal imaging of wild type Elf1-iCas9 cells and Elf1-iCas9 cells transfected with JARID2 sgRNA6.1 (2iL-I-F(C); 5iLA(D)). Blue: DAPI; Green: Oct4, Red: Nanog and Magenta: JARID2 (higher concentration of Ab used for 5iLA to detect lower levels of JARID2: JARID2 Ab 1:200 in 2iL-I-F; 1:100 in 5iLA). The arrows indicate JARID2 KO cells, Scale bar 50 µm (C) or 10 µm (D).