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. 2018 May 18;17(6):671–680. doi: 10.1080/15384101.2017.1415679

Figure 4.

Figure 4.

Effects of the maximum non-cytotoxic doses of tetrandrine on DNA integrity. (A) Double-strand breaks were analyzed by DNA comet assay. Both CNE1 and CNE2 cells exposed to combined treatment of tetrandrine and irradiation exhibited more significant migration of DNA into the tail of the comet compared with cells exposed to irradiation alone. (B) CNE1 cells were exposed to tetrandrine, irradiation (4 Gy), or combined treatment for 24 h. Western blotting was performed to determine the level of γ-H2AX. (C) Quantification of the western blot band intensity for γ-H2AX in CNE1 cells was performed using ImageJ. Each data point represents the mean and SE from 3 experiments (*p<0.05 vs control, #p<0.05 vs 4 Gy). (D) CNE2 cells were exposed to tetrandrine, irradiation (4 Gy), or combined treatment for 24 h. Western blotting was performed to determine the level of γ-H2AX. (E) Quantification of the western blot band intensity for γ-H2AX in CNE2 cells was performed using ImageJ. Each data point represents the mean and SE from 3 experiments (*p<0.05 vs control, #p<0.05 vs 4 Gy).