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. 2018 Apr 2;17(6):759–765. doi: 10.1080/15384101.2018.1426414

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Figure 3. — Decreased linc-GPR65-1 inhibited proliferation, cell motility, invasion and migration of AGS cells. A. The knockdown efficiency of siRNA against linc-GPR65-1 was confirmed using qRT-PCR, and the efficiency was approximately 75%, 50%, 60% after treated with three different siRNA, respectively. And GAPDH is not changing upon lncRNA knockdown when using another internal reference (β-actin). B. Cell proliferation was detected via CCK-8 assays in the si-linc-GPR65-1 group and the negative control group during a period of 5 days. C. Colony formation assays were also performed to estimate the cell proliferation capacity. D.E. Cell cycle and apoptosis rate of AGS cells were evaluated by flow cytometry. F. The invasion (right) and migration (left) of AGS cells were evaluated in the si-linc-GPR65-1 group and the negative control group by transwell assays (magnification × 100).