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. 2018 May 25;13(5):e0197491. doi: 10.1371/journal.pone.0197491

Fig 2. RCAN1 deficiency elevates IκBα phosphorylation in vitro following P. aeruginosa LPS challenge.

Fig 2

Wild-type (+/+) and RCAN1-deficient (-/-) BMMs were stimulated with 200 ng/ml P. aeruginosa LPS for 3 h, 6 h, 12 h and 24 h or left untreated (NT). Cell lysates were subjected to Western blot analysis for phospho- and total IKKα, IKKβ and IκBα, as well as actin as a loading control. Blots are representative of three independent experiments (A). Densitometry analysis of phosphorylated IKKα (B), IKKβ (C) and IκBα (D) was normalized to their total protein respectively (n = 3 ± SEM, *p<0.05, ***p<0.001).