Figure 3.

Voltage dependence analysis of DNA translocations in competition mode. Equal voltages ranging from 400 to 1000 mV were applied to both nanopores. Detection was carried out using 300 pM 10 kbp DNA in 2 M LiCl. (a) Representative current–time traces measured at the two detection channels at the different voltages. (b) Scatter plots of dwell times of double pore events plotted for channel 2 versus channel 1. Distributions are symmetric with respect to the diagonal of the plot (dotted line) meaning that the recorded dwell times are comparable for both channels. (c,d) Voltage dependence of dwell time and peak current for double pore (dark blue) and single pore (light blue) events. Double pore events experience a significant increase in dwell times, compared to single pore events. Both double pore and single pore events show peak currents increasing and dwell times decreasing with increasing voltages. (e,f) Distribution of start (δ₁) and end (δ₂) offsets for the different voltages. The width of the distributions narrows as voltages increase, with δ₁,δ₂ < |1| ms at 400 mV and δ₁,δ₂ < |0.5| ms at 1000 mV.