WP1066 administration after CCI-S reduces phosphorylated STAT3 levels in injured hippocampus. (A) Representative western blots of protein homogenates from whole ipsilateral hippocampus (relative to side of CCI) of mice 6 hours, 24 hours, 48 hours, 1 week and 16 weeks after CCI-S treated with vehicle or WP1066 probed with pSTAT3 and STAT3 antibodies. (B) Quantification of pSTAT3 levels from CCI-S, CCI-S + WP and sham injured controls showed that from 6 hours to 48 hours post injury that pSTAT3 levels were statistically lower than CCI-S injured mice treated with WP1066 compared to vehicle-treated CCI-S mice. * P < 0.05, ** P < 0.01, P < 0.001 (n = 9 for sham, n = 9 for CCI-S and n = 6 for CCI-S + WP at 6 hours, n = 10 for sham, n = 9 for CCI-S and n = 7 for CCI-S + WP at 24 hours and n = 7 for sham, n = 7 for CCI-S and n = 7 for CCI-S + WP at 48 hours and n = 6 for sham, n = 8 for CCI-S and n = 6 for CCI-S + WP at 72 hours and n = 6 for sham, n = 7 for CCI-S and n = 6 for CCI-S + WP at 1 week and n = 6 for sham, n = 7 for CCI-S and n = 7 for CCI-S + WP at 16 weeks).