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. 2018 May 25;4(5):eaar4418. doi: 10.1126/sciadv.aar4418

Fig. 5. Gold label positions for an RNA kink-turn motif.

Fig. 5

(A) Secondary structure of the RNA kink-turn and the two labeling positions at the 3′ ends for the individual constructs. The 3-nt kink-turn bulge is displayed with a small vertical offset, and the three-base mismatch region is shown in orange. (B) Shape reconstruction of the unlabeled RNA kink-turn (light gray shape) superimposed with the high-resolution structure (22). (C) Low-resolution model (gray spheres) and best-fitting gold nanocrystal positions (blue and red spheres) after filtering. (D) Scatter plot of filtered gold label positions. The x axis value of the fitted label positions is plotted versus the overall distance to the geometrical center of the low-resolution shape reconstruction.