Video 2. Actin filaments are exposed to a periodic alternation of a solution of unlabeled actin (0.3 µM actin, 50 mM KCl) for 100 s and a solution of 15% Alexa 488-labeled actin (0.5 µM actin +2 µM profilin, 50 mM KCl) for 20 s.

Download video file ^{(745.2KB, mp4)}

DOI: 10.7554/eLife.34176.004

The filaments are transiently exposed to a solution of 11 nM mDia1(FH1-FH2-DAD) for 5 s, after frame number 5. Images were acquired in epifluorescence while exposing to unlabeled actin. Full field of view is 137 × 137 µm. Interval between images is 120 s (movie is accelerated 360x). The solution flows from left to right. Corresponds to Figure 1B of the main text.