Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (E. coli) | BL21(DE3) | Thermo Fischer | Cat# C600003 | |
Biological sample (Rabbit) | Rabbit muscle | INRA Jouy-en-Josas | N/A | |
Peptide, recombinant protein | Mouse mDia1(FH1-FH2-DAD) | Uniprot | O08808 | seq. 552–1255 aa |
Peptide, recombinant protein | Mouse mDia2(FH1-FH2-DAD) | Uniprot | Q9Z207 | seq. 521–1171 aa |
Peptide, recombinant protein | Human profilin-1 | Uniprot | P07737 | |
Antibody | PentaHis Biotin conjugate | Qiagen | 34440 | |
Chemical compound, drug | Alexa Fluor 488 succinimidyl ester | Life Technologies | Cat#A20000 | |
Commercial assay or kit | Protino Ni-NTA Agarose beads | Macherey-Nagel | Cat#745400.25 | |
Commercial assay or kit | HiLoad 16/60 Superdex 200 gel filtration column | GE Healthcare | Cat#28-9893-35 | |
Software, algorithm | numpy/scipy packages | Python |