Skip to main content
. Author manuscript; available in PMC: 2018 May 25.
Published in final edited form as: Free Radic Biol Med. 2017 Dec 20;115:412–420. doi: 10.1016/j.freeradbiomed.2017.12.019

Fig. 6. NOX1 was up-regulated by palmitic acid in cultured liver sinusoidal endothelial cells.

Fig. 6

(A) Expression of NOX isoform mRNAs in freshly isolated liver sinusoidal endothelial cells (LSECs) relative to enriched hepatocytes (left). N=3 per group. Representative photographs of hepatocytes and LSECs cultured for 24 h (right). (B, C) Time- and dose-dependent expression of NOX1 mRNA in primary cultured LSECs. Cells were incubated with palmitic acid (PA) for 24 h (B), or with 400 μM PA (C). (D) Expression of NOX1 mRNA in the presence of 400 μM PA or 400 μM oleic acid (OA) for 24 h. LSECs were pretreated with 10 μM TAK242 before PA treatment. (E) Expression of NOX2 and NOX4 mRNAs in LSECs treated with PA for 24 h. N=3–12 per group. *p < 0.05, **p < 0.01, #p < 0.01.