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. Author manuscript; available in PMC: 2019 Jan 25.
Published in final edited form as: Cell. 2018 Jan 25;172(3):590–604.e13. doi: 10.1016/j.cell.2017.12.032

Figure 1. G3BP1-APEX2 Mediates Specific Biotinylation of Stress-Granule-Associated Proteins.

Figure 1

(A) Schematic of APEX proximity labeling to tag SG proteins with biotin.

(B) Streptavidin staining of unstressed and NaAsO2-treated HEK293T G3BP1-APEX2-GFP and hPGK-NES-APEX2-GFP cells. Scale bars, 25 μm.

(C) Streptavidin-HRP western blot analysis of induced protein biotinylation in lysates from NES-APEX2-GFP and G3BP1-APEX2-GFP cells.

(D) Schematic of G3BP1 interactome changes upon stress.

(E) Experimental designs for detecting the G3BP1 interactome changes under different conditions, including log2 H/L ratio distributions of all proteins detected, overlaid with log2 H/L ratio distributions of known SG proteins.

See also Figures S1 and S2 and Table S1.