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. 2001 Sep;12(9):2629–2645. doi: 10.1091/mbc.12.9.2629

Figure 10.

Figure 10

Potentiation of DCA-induced apoptosis by MAPK inhibition correlates with reduced protein expression of c-FLIP-L, c-FLIP-S and Bcl-XL and is blocked by overexpression of c-FLIP-s. Primary rat hepatocytes were infected with poly-l-lysine–conjugated adenoviruses to express either c-FLIP-S or a CMV null virus. Twenty-four hours after infection, cells were treated with DCA (50 μM) and either vehicle control or PD98059 (50 μM), and the percentage of apoptosis determined 360 min after addition of H-33342. Data are the means of three experiments ± SEM. (Inset) DCA increases protein expression of Bcl-XL, c-FLIP-S, and c-FLIP-L in primary hepatocytes, which is reduced by MAPK inhibition. Cells were treated with DCA (50 μM) and either vehicle control or PD98059 (50 μM), and the protein levels Bcl-XL and c-FLIP isoforms were determined 120 min after addition. Data are a representative experiment (n = 3).