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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Diagn Microbiol Infect Dis. 2018 Feb 9;91(2):123–125. doi: 10.1016/j.diagmicrobio.2018.02.002

Figure 1. MGMR PCR limit of detection (LOT) and melting peak analysis.

Figure 1

A) The limit of detection was determined by testing the 1:10 serial dilution of MG 37 (ATCC 33530) genomic DNA. Each diluent was tested in triplicates. The starting concentration was 2.3 x 106 genome copy (GC)/μL in PCR template. The LOD was 2.3 GC/μL in PCR template, or ~5 GC/test. The PCR efficiency was 1.746. B) The representative melting peaks of 5 known mutations associated with MG macrolide resistance.