Figure 3.
RNA-dependent RNA synthesis. (A) RNA synthesis on the WT template. The RNA corresponding to the 157 last nucleotides (Fig. 1A) was used as template in the RdRp assays with 3H-UTP labelled nucleotide. The amount of radioactivity incorporated into the nucleic acids synthesized was measured after 2 hours at 30 °C and expressed as the mean and standard deviation of the ratio to the WT. The experiment was performed in the presence of 40 mM NaCl, 40 mM KCl, or 40 mM NaCl plus 0.5 µM PhenDC3. (B) 5BSL3.2 sequence or PolyrA RNA in the presence of oligo(U) primer were used as templates in the conditions described in A. (C) Comparison of RNA synthesis on WT and mutated template (MutG4). (D) MutG4 template was used in RNA synthesis assay as described in A. For each conditions: n > 3 independent experiments in duplicate/error bars, mean ± SEM. Student test: no indication if p > 0.05; **p < 0.0005 and ***p < 0.00005.