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. 2018 May 25;8:8162. doi: 10.1038/s41598-018-26546-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR Adipogenesis— the expression of adipogenic genes Adiponectin (AN), PPARg, and Leptin (Lep) for short-term (3–7 years) frozen-thawed ASCs, long-term (> = 10 years) frozen-thawed ASCs, and fresh ASCs. The donor numbers (1–9) shown on the x-axis correspond to the numbers shown in Table 1. The y-axis shows the mRNA fold change normalized to CycB as described in the text with each donor measurement being made in triplicate (corresponding to the error bars shown). No significant differences in gene expression between long-term and short-term frozen groups in relation to the fresh ASCs is observed.