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. 2018 May 25;8:8108. doi: 10.1038/s41598-018-26326-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tilted-field scan enabled by rapid focusing of the EWTL lens. (A) Maximum intensity projection of a thick coronal section of mouse brain, expressing GCaMP6s in neurons, acquired by the 2P-FCM. Arrows indicate cell bodies that are visible during tilted-field scanning. (B) Side (XZ) projection of the image volume in A. The same cell bodies are indicated by the arrows. The planes for the horizontal and angled scan limits are indicated and color coded red for the −30 degrees scan, and white for the +30 degrees scan. (C) Images showing the tilted-field scans, indicating the same cell bodies that are shown to intersect with the red or white planes in B.