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. 2018 May 25;8:8108. doi: 10.1038/s41598-018-26326-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mouse implant and verification of GCaMP6s expression. (A) Attachment mechanism of 2P-FCM onto baseplate attached to the mouse skull. (B) Photo of a behaving mouse with 2P-FCM attached. (C) Verification of stability of imaging FOV over 17 days, showing the same blood vessels with epifluorescence measured through the FCM. (D) Confocal image of the neocortex for a fixed cortical coronal slice of the mouse’s brain 12 weeks post-injection of AAV5 expressing GCaMP6s driven under control of the synapsin promoter.