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. 2018 May 25;8:8121. doi: 10.1038/s41598-018-26445-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Boxplots of PELE steps required for ligand release from AAO and its Phe397 variants. (a) p-Anisic acid. (b) p-Anisaldehyde. Number of PELE steps is directly related to time. Grey, native AAO; blue, F397W; red, F397Y; yellow, F397A; and light green, F397L variants. Boxes contain 50% of the results (two quartiles), horizontal lines inside them indicate the mean value; while the upper and lower whiskers (vertical lines) contain the remaining quartiles (50%). The overall height of boxes (whiskers included) is indicative of the spread of the results. Isolated points represent results significantly different from the rest of data values.