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. 2018 May 25;8:8100. doi: 10.1038/s41598-018-26516-z

Figure 4.

Figure 4

Inhibition by viperin is not affected by T7-polymerase localization. (a) Intracellular localization of the NLS-T7-polymerase. Fluorescent microscopy images of BHK-21 cells co-transfected with T7-GFP and either T7-polymerase or NLS-T7-polymerase. Images were obtained 48 h post-transfection Bar = 10 µM. (b) The effect of viperin on NLS-T7 polymerase. BHK-21 cells were co-transfected with viperin, T7-GFP and either T7 polymerase or NLS-T7-polymerase. The cells were analyzed by Western blot using the indicated specific antibodies 48 h post transfection. (c) HEK293-T7 cells were transfected with viperin or a control plasmid as indicated. Cell lysates were subjected to immunoprecipitation 48 hours later, using A/G agarose beads and viperin specific antibody. Western blot analysis was then performed using anti-Viperin and anti-T7 antibodies. Full-length blots are presented in Supplementary Fig. 4.