Figure 5.

Viperin inhibits T7 polymerase mediated RNA synthesis. (a) BSR-T7 cells were infected with bicistronic lentiviruses co-expressing either viperin or luciferase and RFP as a transfection control. The cells were transfected with T7-GFP 48 h post-infection and with BrU after additional 24 h. (b) A graph presenting cytosolic vs nuclear fluorescence intensity in vector transfected or viperin transfected individual cells. (c) Quantification of the results presented in B. Results are mean ± standard error of the cytoplasm to nuclear fluorescence intensity ratio from 31 cells in each treatment (***p < 0.0001, Student t-test). Bars = 20 µM.