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. 2018 May 25;8:8171. doi: 10.1038/s41598-018-25837-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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M1 and M2 macrophages (MΦs) in wild type (WT) mice ovary. (a) Representative flow cytometry analysis of M1 and M2 MΦs in the WT mice ovary. M1-like MΦ was defined as CD45+/F4/80+/CD11c+/CD206− cells, and M2-like MΦ was defined as CD45+/F4/80+/CD206+/CD11c− cells and Dendritic cells (DCs) was defined as F4/80− CD11c+ cells. (b) The proportion of CD11c+F4/80+ cells, M1-like MΦs (left panel) and CD206+ F4/80 cells, M2-like MΦs (middle panel) and F4/80− CD11c+ cells, DCs (right panel) in ovary. The proportion of M1-like MΦs significantly increased following follicular induction with PMSG 48 h, while that of M2-like MΦs and DCs was not increased. The data are shown as the means ± standard error of the mean (SEM). A P-value of < 0.05 was considered statistically significant by Mann-Whitney U test. N.S; not significant compared to WT mice. n; the number of mice.