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. 2018 May 25;8:8171. doi: 10.1038/s41598-018-25837-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The involvement of CD11c+ cells, M1 macrophages (MΦs) and dendritic cells (DCs) in folliculogenesis. (a) The Hematoxylin Eosin (HE) staining of ovaries in follicular induction with PMSG 48 h in Wild Type (WT) (left Panel) and CD11c diphtheria toxin receptor (DTR) mice (middle). (b) The Ki-67 staining, a cell proliferation marker, in follicular induced ovary with PMSG 48 h in WT and CD11c DTR mice. Negative control data are also shown. (c) The number of each follicle stage in follicular induction in WT and CD11c DTR mice. We counted the number of follicles at each stage, such as atresia (At), primordial (P1), primary (P2), secondary (S), antral (An), and corpus luteum (CL) in CD206 DTR (right panel) and WT (left panel) mice, as previous described³². (d) The serum estradiol levels after 48 h PMSG in WT and CD11c DTR mice. The data are shown as the means ± SEM. A P-value of < 0.05 was considered statistically significant by Mann–Whitney U test. n; the number of mice. N.S: not significant compared to WT.