Fig. 7.

Increasing Cbx7 levels inhibits the proliferation of patient-derived DIPG cells. a Immunostaining of Cbx7 in 9427 and SF8628 cells carrying transgenic Cbx7 under the control of TRE-tight promoter. The expression of Cbx7 was induced by a variety of doxycycline concentrations. Scale bar, 2.0 µm. b Quantification of fluorescence intensity of Cbx7 from a. c Representative images of proliferation response of 9427 and SF8628 cells to increasing levels of Cbx7. Images of cells treated with 0.1 and 0.5 µg per ml of Dox are not shown. Scale bar, 1000 µm. d Quantification of proliferation response of 9427 and SF8628 cells to increasing levels of Cbx7. Values shown are the average from triplicate samples for each incubation condition