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. 2018 May 25;7:95. doi: 10.1038/s41426-018-0093-2

Fig. 1. GTV isolation from ticks and EM analysis of viral particles.

Fig. 1

a Immunofluorescence assays to survey SFTSV infection in Vero cells from each passage. The images taken from different passages showing the virus proliferation derived from the 1:40 dilution are presented. Cells immunostained by green fluorescence indicated that they were infected by GTV. P1, the first passage; P3, the third passage; P5, the fifth passage; and P7, the seventh passage. b EM analysis of GTV particles purified from the cell culture supernatants. The supernatants were harvested from Vero cells infected with GTV after 5 days p.i. and were centrifuged and filtered to remove the cell debris. The clarified supernatants were subjected to ultracentrifugation. The fractions containing viral particles were harvested and used in negative-staining EM analyses. c The image obtained by TEM show that virus particles were located in cytoplasm of infected Vero cells. The enlarged images of interest show virus particles from (b) and (c) are shown on the right. N, nucleus; C, cytoplasm; and NM, nuclear membrane