Fig. 4. Reporter assays using the SFTSV replicon system with the substitution of SFTSV, GTV, or HRTV NP, respectively.

The reporter assays were performed using the egfp reporter plasmid system. NP expression in each test was immunostained with α-SNP or an HA tag. Cell nuclei were stained with Hoechst 33258. The eGFP expression was visualized under an inverted fluorescence microscope