Fig. 6. GTV infection suppresses type I IFN signaling.

a GTV infection inhibits IFN-β-triggered activation of the ISRE promoter. 293T cells were co-transfected with the IFN-β reporter and pRL-TK plasmids. At 12 h post transfection (p.t.), cells were mock infected or infected with SFTSV or GTV. After 8 h, cells were treated with SeV for another 16 h, after which luciferase activities were measured. The relative luciferase activities (Rel.Lucif.Act.) were expressed as the firefly luciferase activity normalized to Renilla luciferase activity. b GTV infection suppressed IFN-induced gene expression. 293T cells were mock infected or infected with SFTSV or GTV for 24 h and then treated with IFN-β (200 U/ml) or left untreated for 10 h. Expression of ISGs was measured by qRT-PCR. Each test was performed in triplicate. Data are presented as the means ± SD