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. 2018 May 25;8:8157. doi: 10.1038/s41598-018-26383-8

Figure 1.

Figure 1

Preventive effect of OCA on the development of NASH in MC4R-KO mice. (a) Experimental protocol for examination of the preventive effect of obeticholic acid (OCA) on the development of NASH in melanocotin-4 receptor deficient (MC4R-KO) mice fed Western diet (MC4R-WD). Vehicle, 0.5% carboxymethyl cellulose (CMC). Wild type (WT) mice on standard diet (WT-SD) treated with the vehicle were used as the control group. (b) Growth curve of WT and MC4R-KO mice. Open circle, WT-SD treated with the vehicle (n = 7); closed circle, MC4R-WD treated with the vehicle (n = 10); open and closed square, MC4R-WD treated with OCA at 3 mg/kg (n = 10) and 10 mg/kg (n = 9), respectively. (c) Hematoxylin and eosin staining of the liver after vehicle or OCA treatment. Histological analysis using non-alcoholic fatty liver disease (NAFLD) activity score (NAS). (d) Evaluation of liver fibrosis using Sirius red staining. (e) Serum concentrations of collagen type IV and soluble vascular cell adhesion molecule-1 (VCAM-1). (f) Hepatic mRNA expression levels of collagen type IV and VCAM-1. Scale bars, 100 μm. CV, central veins. V, Vehicle, n.d., not detected. ##p < 0.01; *p < 0.05, **p < 0.01 vs. vehicle-treated MC4R-WD group.

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