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. 2018 May 25;9:2069. doi: 10.1038/s41467-018-04439-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — CaMKIIα binding to GluN2B is critical for basal AMPAR transmission. a Timeline of the experiment. b Scheme of the structural organization of CaMKIIα with the point mutations indicated (K42R dark green; F98K blue; I205K light green). c−e Scatterplots showing amplitudes of AMPAR EPSCs for single pairs (open circles) of control and transfected cells of DKO + CaMKIIα F98K (c, n = 14 pairs), DKO + CaMKIIα I205K (d, n = 23 pairs), and DKO + CaMKIIα K42R_I205K (d, n = 14 pairs). Filled circles indicate mean ± SEM (c, Control = 111 ± 19.9; DKO + CaMKIIα F98K = 44.33 ± 11.8, p = 0.002; d, Control = 106.8 ± 8.1; DKO + CaMKIIα I205K = 46.5 ± 8.6, p < 0.0001; e, Control = 112.5 ± 9; DKO + CaMKIIα I205K_K42R = 49.5 ± 7.5, p < 0.0001). f Bar graph of ratios normalized to control (%) summarizing the mean ± SEM of AMPAR EPSCs of values represented in c (40 ± 1.1, p < 0.0001), d (45.5 ± 7.2, p < 0.0001) and e (44.6 ± 5.6, p < 0.0001). DKO data (red bar) from Fig. 1l and DKO + CaMKIIα (light green) from Fig. 3e values are included in the graph. g–i Scatterplots showing amplitudes of NMDAR EPSCs for single pairs (open circles) of control and transfected cells of DKO + CaMKIIα F98K (g, n = 14 pairs), DKO + CaMKIIα I205K (h, n = 23 pairs), and DKO + K42R_I205K (i, n = 14 pairs). Filled circles indicate mean ± SEM (g, Control = 36.2 ± 7.7; DKO + CaMKIIα F98K = 35.3 ± 6, p = 0.49; h, Control = 59.3 ± 5.4; DKO + CaMKIIα I205K = 53.2 ± 6.4, p = 0.20; i, Control = 62.8 ± 8.5; DKO + CaMKIIα I205K_K42R = 54.9 ± 11.4, p = 0.18) j Bar graph of ratios normalized to control (%) summarizing the mean ± SEM of NMDAR EPSCs of values represented in g (101 ± 14, p = 0.62), h (91.1 ± 7.3, p = 0.74) and i (88.2 ± 10.2, p = 0.66). DKO data (red bar) from Fig. 1p and DKO + CaMKIIα (light green bar) from Fig. 3h values are included in the graph. Raw amplitude data from dual cell recordings were analyzed using Wilcoxon signed rank test (p values indicated above). Normalized data were analyzed using a one-way ANOVA followed by the Mann−Whitney test (***p < 0.0001; **p < 0.001). Scale bars: 50 ms, 50 pA