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. 2018 May 25;8:8125. doi: 10.1038/s41598-018-25991-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Multiple views from a single scan of an adult zebrafish. (A) Representative oblique slice defined by three landmarks (anterior and posterior end of the bulbus arteriosus, the centre of the 1st vertebra). (B) Inset of Fig. 2A, showing the ventricle (ven), bulbus arteriosus (ba), as well as the distance measurements taken. (C) Three-dimensional volume rendering of the bulbus arteriosus (purple) and ventricle (green). V, first vertebra. ba, bulbus arteriosus. ven, ventricle. Landmark 1, bulbus junction with the first branchial arch. Landmark 2, ventricle – bulbus valve. Landmark 3, ventricle apex. Landmarks 4 and 5 define the widest distance of the ventricle, perpendicularly to ventricle length (D_2–3). D_1–2, Bulbus-arteriosus length (BaL); D_2–3, Ventricle length (VL); D_4–5, maximum ventricle depth (VD), perpendicular to VL.