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. 2018 May 25;8:8181. doi: 10.1038/s41598-018-26079-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative proteomic analysis of meropenem resistant and susceptible Achromobacter xylosoxidans clinical isolates. (A) Tryptic digests of protein extracts of the meropenem resistant (AchroR, Table 1) and susceptible (AchroS, Table 1) isolate were analysed by LC-MS/MS. Spectra were assigned to peptides based on database searching. Identified spectra were then assigned to the corresponding proteins and the total number of spectra per protein were counted. Each circle represents one protein with the number of spectra observed in the resistant and the susceptible isolate. Hence, proteins on the diagonal were observed in similar counts in both isolates. Axc (arrow), a classA PenP-family beta-lactamase, is the most prominent outlier. See also Table S2 and Supplemental Fig. S2. (B) The full amino acid sequence of Axc, with the peptides identified by LC-MS/MS analysis underlined. Conserved residues from serine beta-lactamases, Ser-X-X-Lys, Ser-Asp-Asn and the active site Glu, are in bold³⁷. (C) Extracted ion chromatograms of m/z values corresponding to tryptic peptides of Axc in the meropenem resistant isolate (AchroR, upper panel) and susceptible isolate (AchroS, lower panel). The corresponding tryptic peptides are indicated above the corresponding peaks.