Figure 1.

Overview of experimental setup. (A) Flowchart of protocol: vascular tissue cubes were cut into sections using a vibrating blade microtome (Leica VT1200S). Tissue sections were cultured in supplemented culture medium in 24-well plates in a dark humidified atmosphere at 37 °C in 5% CO₂. (B) Photograph of self-designed 3D-printed mould in which tissue cubes are fixed in agarose. (C) Photograph of vibratome Leica VT1200S, used for tissue sectioning. α-SMA indicates alpha smooth muscle actin; FCS, fetal calf serum and STED, stimulated emission depletion.