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. 2018 May 25;8:8094. doi: 10.1038/s41598-018-26549-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence images at 10× magnification using Zeiss Axiovert 200 M Marianas™ Microscope. Human tissue stained with LIVE/DEAD® Viability/Cytotoxicity Kit. Green fluorescence shows live cells, while red fluorescence indicates dead nuclei. (A) Alive tissue at day 62 after harvesting. Tissue viability of 58%. (B) Alive tissue at day 62 after harvesting at 2.5× magnification and (C) at 40× magnification. Outgrowth of new cells is observed after 92 days, while original tissue shows only staining of EthD-1 (dead cells). Tissue viability of 85%. EthD-1 indicates ethdium homodimer-1.