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. 2018 May 25;8:8094. doi: 10.1038/s41598-018-26549-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence images showing aneurysmal aortic wall sections. (A–C) XY stitched images at 40× magnification using Nikon A1R confocal microscope on day 0, 7 and 14 after culturing. Smooth muscle cells are immunostained by smoothelin (white), actin (found in practically all eukaryotic cells) by phalloidin (red) and cell nuclei by DAPI (blue). Smooth muscle cells are found in the media and other cells (mainly fibroblasts) in the adventitia. M indicates media; A, adventitia and DAPI, 4′,6-diamidino-2-phenylindole.