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. 2018 May 25;8:8094. doi: 10.1038/s41598-018-26549-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence images at 10× (A,B) and 63× oil (C) magnification using Zeiss Axiovert 200 M Marianas™ Microscope. (A) After 14 days of culturing, tissues were enzymatically digested using collagenase. Live separate cells, stained with Calcein AM (green), float as round cells in culture medium. (B) After 24 hours of additional culturing, the cells were attached to the slide. (C) Different cell type characteristics and differences in cell nuclei are observed in attached cells. Live cytoplasm is stained with Calcein AM (green) and cell nuclei are stained with DAPI (blue). AM indicates acetoxymethyl and DAPI, 4′,6-diamidino-2-phenylindole.