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. 2018 May 25;9:2061. doi: 10.1038/s41467-018-04523-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Mammalian sperm zinc signature. a–d Epifluorescence microscopy of boar sperm zinc signature (green). e–h Epifluorescence microscopy of bull sperm zinc signature. i–l FlowSight image gallery of human sperm zinc signature (scale bar: 20 μm). m Boar spermatozoa after 72 h of storage in Beltsville thaw solution (BTS semen extender) show varied zinc signatures. Imprecise fluorescent to bright-field overlay illustrates motile status (all scale bars: 25 μm, unless noted)