Figure 6.

(A) In vitro aminoacylation of deacylated mitochondrial tRNA fractions from M. domestica with lysine, followed by periodate oxidation of nonprotected molecules. Lane nox designates mitochondrial tRNAs that were labeled without prior aminoacylation or oxidation. Lane ox indicates that mitochondrial tRNAs were oxidized quantitatively with a 1000-fold molar excess of NaIO₄ so that no molecules could be labeled at the 3′-end. Mitochondrial tRNA fraction of lane K was aminoacylated in vitro with lysine before oxidation. Aminoacylated, oxidation-resistant tRNAs were deacylated and 3′-end labeled. The band indicated by the asterisk was isolated for sequence analysis (two reactions) and identified as a nuclear type of tRNA^Lys_(TTT). (B) Alignment of the human nuclear tRNA^Lys and the obtained sequence of the marsupial tRNA^Lys associated with mitochondria. The alignment was done according to Corpet (1988). Human cyt, human cytosolic tRNA^Lys; Monodelphis mt, mitochondria-associated tRNA^Lys in Monodelphis; Y = C or U; W = A or U.