Table 1.
Serum protein levels of cytokines
| Cytokine | |||||||
|---|---|---|---|---|---|---|---|
| IL-1β | IL-6 | TNF-α | IL-17a | IFN-γ | IL-10 | ||
| Short-term treatment | CTR + vehicle | 123.7 ± 65.7 | 9.9 ± 1.8 | 481.2 ± 93.0 | 22.0 ± 4.8 | 26.4 ± 4.7 | 61.4 ± 11.2 |
| MPTP + vehicle | 180.8 ± 36.5 | 11.7 ± 2.2 | 500.8 ± 104.2 | 39.0 ± 17.7 | 27.3 ± 5.3 | 68.6 ± 13.3 | |
| CTR + indomethacin | 255.2 ± 30.2 | 11.6 ± 2.0 | 537.2 ± 91.4 | 27.1 ± 4.4 | 29.9 ± 5.1 | 76.4 ± 12.1 | |
| MPTP + indomethacin | 157.5 ± 76.5 | 9.1 ± 1.3 | 442.8 ± 91.5 | 22.8 ± 2.1 | 26.2 ± 1.8 | 70.4 ± 4.3 | |
| Long-term treatment | CTR + vehicle | 94.0 ± 36.0 | 8.6 ± 1.1 | 406.3 ± 53.7 | 15.5 ± 2.2 | 23.9 ± 3.0 | 54.7 ± 7.1 |
| MPTP + vehicle | 151.4 ± 29.6 | 11.5 ± 1.9 | 569.5 ± 82.3 | 38.2 ± 6.4** | 30.0 ± 3.9 | 88.6 ± 9.7* | |
| CTR + indomethacin | 92.0 ± 35.1 | 10.7 ± 1.7 | 466.8 ± 89.9 | 24.6 ± 4.9 | 27.0 ± 4.3 | 60.1 ± 10.2 | |
| MPTP + indomethacin | 126.9 ± 26.7 | 9.4 ± 1.3 | 354.8 ± 73.6 | 19.3 ± 3.6## | 24.8 ± 4.2 | 52.5 ± 9.9## | |
Multiplex ELISA was performed to evaluate the levels of pro-inflammatory IL-1β, IL-6, TNF-α, IL-17a, and IFN-γ and anti-inflammatory IL-10 in serum of short-term and long-term groups. Values are expressed as mean ± SEM in pg/ml, n = 7–11/group. A two-way ANOVA with factors neurotoxin, drug, and their interaction was performed
CTR control, MPTP 1-methyl-4-(2′-methylphenyl)-1,2,3,6-tetrahydropyridine hydrochloride
A significant interaction was followed by Bonferroni post hoc test: *p ≤ 0.05, **p ≤ 0.01 compared to CTR + vehicle; ##p ≤ 0.01 compared to MPTP + vehicle