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. 2018 May 25;18:44. doi: 10.1186/s12866-018-1185-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Heat stress induced cytokine secretion. Expression of cytokines Il6, Il1b, Tnf, Mcp1, Ifng and Il10 by mouse splenic cells with and without exposure to heat quantified by RT-PCR (a). Quantification of cytokines: TNF (b), IL-6 (c), IL-1β (d), MCP-1 (e), and IFN-γ (f) in the sera of mice by flow cytometer and sandwich ELISA. Data represent the mean ± SD of replicates of six samples. Asterisks indicate significant differences (p < 0.05)