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. 2018 May 25;18:44. doi: 10.1186/s12866-018-1185-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Heat stress caused an increase in NF- κB and iNOS expression in splenic cells. The expression of heterodimers of NF- κB: p50, p52 and p65 from splenic cells was quantified by RT-PCR (a). Immunoblot analysis of the expression of heterodimers of NFkB: p50, p52 and p65 from splenic cells (b). Immunoblot analysis of iNOS expression following heat exposure (c). Asterisks indicate significant differences (p < 0.05)