Figure 1. Chronological flowchart of fecal DNA preservation experiment.

Fecal samples from one canine donor were collected, aliquoted, and treated with no buffer (P), RNA later (R), 70% ethanol (E), and 50:50 Glycerol:PBS (G). After 2 h of incubation at 25 °C, Day 0 samples were immediately processed for DNA extraction, while other samples were stored at the indicated temperatures (room temp: 25 °C, refrigeration: 4 °C, freezing: −80 °C) until extractions on Days 7, 14, and 56.