Table 1. Sequences of primers for PCR/OE-PCR.
Primer name | Primer sequence (5′-3′) |
---|---|
MS2-F | CGggatccGTGCGAGCTTTTAGTACCCTTGA |
MS2-R | CCCaagcttTGTTGTCTTCGACATGGGTAATCCTC |
IN-F | CTGACCAACGTGCGTGGCGATCTGCAAGTCCTGGCACAGAAAGCTGCACGTCCTCTGCCTACTGGCGACGTGACTGTCGCCCCAAGCAA |
IN-R | TGCCAGGACTTGCAGATCGCCACGCACGTTGGTCAGGGAACCCTCAGCGTACTTGCAGTTCCCGTTTCCGCCATTGTCGACGAGAACGAAC |
Notes.
Lowercase part in primers MS2-F and MS2-R represent for restriction enzymes (Bam H I and Hin d III).
The sequences in bold type in two primers IN-F and IN-R were the epitope sequence 131–160 of VP1, and underlined 36 nucleotides were reverse complementary.