Table 1. Sequences of primers for PCR/OE-PCR.

Primer name	Primer sequence (5′-3′)
MS2-F	CGggatccGTGCGAGCTTTTAGTACCCTTGA
MS2-R	CCCaagcttTGTTGTCTTCGACATGGGTAATCCTC
IN-F	CTGACCAACGTGCGTGGCGATCTGCAAGTCCTGGCACAGAAAGCTGCACGTCCTCTGCCTACTGGCGACGTGACTGTCGCCCCAAGCAA
IN-R	TGCCAGGACTTGCAGATCGCCACGCACGTTGGTCAGGGAACCCTCAGCGTACTTGCAGTTCCCGTTTCCGCCATTGTCGACGAGAACGAAC

Notes.

Lowercase part in primers MS2-F and MS2-R represent for restriction enzymes (Bam H I and Hin d III).

The sequences in bold type in two primers IN-F and IN-R were the epitope sequence 131–160 of VP1, and underlined 36 nucleotides were reverse complementary.