Figure 6.

Treatment of cultured B35 neuroblastoma cells with palladin antisense results in impaired neurite outgrowth. (A) B35 cells were transfected with GFP plus control (sense) construct or GFP plus palladin antisense construct and then fixed and stained for palladin. Arrows point to transfected cells. Although antisense transfection led to a reduction in palladin immunoreactivity (right), transfection with the control construct did not (left). (B) B35 cells were transfected with GFP alone, GFP plus empty vector, GFP plus sense construct, or GFP plus palladin antisense construct. The top row of images shows undifferentiated control cells, and the second and third rows show cells stimulated with 1 mM Bt2cAMP. Note that cells treated with palladin antisense failed to extend neurites in response to Bt2cAMP (far right).