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. 2001 Sep;12(9):2730–2741. doi: 10.1091/mbc.12.9.2730

Figure 4.

Figure 4

TGFβ affects in parallel collagen lattice contraction and α-SMA expression. (A) SCFs and LFs were grown in attached collagen gels in serum-containing medium and treated with 10 ng/ml TGFβ1 or TGFβ-sR (1.0–100 ng/ml) for 5 d. Stressed lattices were then released and maximum contraction was recorded after 30 min. TGFβ1 enhanced SCF contraction but had no effect on that of LFs. TGFβ-sR decreased LFs contraction dose-dependently but did not affect that of SCFs. Each bar represents mean values (±SD) of five independent experiments performed with five lattices per condition, respectively. *p ≤ 0.005, **p ≤ 0.001 compared with the respective control. (B) The level of α-SMA expression in collagen gels was quantified by Western blotting at each condition (lanes 1–3: SCFs; 4–8: LFs; lanes 1 + 4: control; 2 + 5: 10 ng/ml TGFβ1; 6–8: 1.0–100 ng/ml TGFβ-sR). No changes were observed when blots were evaluated for total actin.