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. Author manuscript; available in PMC: 2018 Nov 16.
Published in final edited form as: Nature. 2018 May 16;557(7706):570–574. doi: 10.1038/s41586-018-0121-3

Extended Data Figure 3. CHIKV infectivity in CHO-K1 and CHO-745 cells in the presence or absence of ectopic Mxra8 expression.

Extended Data Figure 3

a. Surface expression of Mxra8 on CHO-K1 (WT) and CHO-745 (glycosaminoglycan deficient15) cells stably transduced with control (vector-only) or mouse Mxra8 as judged by flow cytometry. b. Confirmation of HS expression on the surface of CHO-K1 (WT) and CHO-745 cells. Surface expression of HS was evaluated using the R17 protein of rodent herpesvirus Peru, which binds to glycosaminoglycans on the surface of cells38. R17GAG2 is a mutant form of the protein that lacks binding to glycosaminoglycans and served as a negative control. For a and b, data are representative of two experiments. c. CHO-K1 (WT) and CHO-745 cells were transduced stably with control (vector) or mouse Mxra8 and inoculated with CHIKV (strains 181/25, AF15561, or LR-2006) and processed for intracellular E2 protein staining by flow cytometry. Data are from three experiments: mean ± SD (n = 6, one-way ANOVA with a Dunnett’s multiple comparison test, ****, P < 0.001).