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Two-hybrid interactions between the Dnm1p GTPase and C-terminal mutant GED domains
| GTPase1–343 | GTPaseK41A | GTPase1–363 | GTPase1–403 | |
|---|---|---|---|---|
| Insert B-AH/GED | ++* | − | − | − |
| Insert B-AH/GEDK705A | − | − | − | − |
| Insert B-AH/GEDR736A | − | − | − | − |
−, no growth on selective medium; and ++, <50 colonies on selective medium.
Growth observed only for one of the reciprocal AD and BD plasmid pairs. All constructs paired with empty BD and AD plasmids did not grow on selective medium.
