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. 2017 Nov 4;53(6):725–739. doi: 10.1007/s00535-017-1408-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Overexpression of Smad4 could reverse the effect of miR-324-3p on GC cells. a Smad4 protein level was detected by western blot. b Smad4 could reverse the effect of miR-324-3p on cell proliferation. c Smad4 could reverse the effect of miR-324-3p on cell vitality. d Restoration of Smad4 inhibited colony formation. e Flow cytometric assay was performed to verify that Smad4 could reverse the effect of miR-324-3p on cell apoptosis. f Transwell migration assay demonstrated that cell migration ability was restrained by overexpression of Smad4