Fig. 5.

miR-324-3p promoted growth of GC cells in vivo. a, b Nude mice injected subcutaneously with transfected GC cells were killed and the tumors of the mimics group had a larger volume. c, d Tumors of inhibitor group had smaller volume than the NC group. e The results of ki-67 staining assay showed that miR-324-3p promoted proliferation of GC cells in nude mice. f Overexpression of miR-324-3p could inhibit cell apoptosis while knockdown of miR-324-3p promoted cell apoptosis in vivo by TUNEL assay. g, h Smad4 expression had a negative correlation with miR-324-3p in vivo by immunohistochemistry staining and western blot