Figure 4. Traf6 Is Required for Maintaining HSC Quiescence.

(A) TUNEL positive VavCre⁺ or Traf6^−/−VavCre⁺ Lin- and Lin+ BM cells (n = 4 per group).

(B) Representative cell-cycle analysis of VavCre⁺ or Traf6^−/−VavCre⁺ CD150⁺LSK cells.

(C) Proportion of VavCre⁺ or Traf6^−/−VavCre⁺ CD150⁺LSK cells (left) or LSK cells (right) in G0, G1 and S/G2/M phase (n = 4 per group).

(D) Frequency of BrdU⁺ staining in VavCre⁺ or Traf6^−/−VavCre⁺ CD150⁺LSK cells (left) or LSK cells (n = 4 per group). *p < 0.05. Data are represented as mean ± SEM.